Highlights of the protocol include using a hemolytic fixative for sample preparation and disposable base molds for cell block construction RESULTS The cell block failure rate in the current study decreased from 18 with the HistoGel Tube method January 2014December 2014 to 6 with the CellGel method January 2015December 2016
Novel Modification of HistoGelBased Cell Block Preparation Method
PDF Microsoft Word Histogel Processing of Histology and Cytology
HISTOGEL protocol This protocol is designed for processing organoids grown in chamber slides 4 or 8 well The processed histogel blocks will be used for paraffin embedding and generation of sections that contact organoid slices Materials 1 Histogel Thermo Scientific Cat HG4000012 2 05 Eosin in 70 Ethanol 3
Collecting Organoids and placing into Histogel Protocolpdf Size 882 KB Biospecimens Cell General Diagnoses General Topics Biospecimen Processing fixation embedding in Histogel and storage of organoids This SOP is a product of the University of Arizona Cancer Center Tissue Acquisition and CellularMolecular Analysis Shared
PDF Histogel Protocol Cancer
HistoGel Vial 10mL CS12 Cancer Diagnostics Inc
HistoGel Specimen Processing Gel Instructions Cancer Diagnostics
Histogel Protocol Cancer
PDF Special Techniques Cell Pellet Protocol National Institute of
HistoGels formulation outperforms other agarose gel mixtures or thromboplastin at a fraction of the cost HistoGel will not retain histological stains eliminating unwanted discoloration around specimens on slides Cancer Diagnostics Inc is an ISO 13485 Accredited and FDA cGMP Compliant Company Login Register Request a Quote
Improvements in cell block processing The CellGel method
swirling motion OR allow HistoGel to settle to the bottom of tube 3 Allow HistoGel to solidify by cooling to near room temperature 20 C This can be achieved by use of a cooling plate ice cubes freeze pack or allowing to cool naturally 4 Remove HistoGel pellet containing the specimen and place inside a HistoScreen Tissue Cassette 5
Collecting Organoids and placing into Histogel Protocol
Protocol to optimize the biobanking of ovarian cancer organoids by
Histogel Protocol Cancer
The described protocol has 2 potential limitations that should be acknowledged One is a longer cell block processing time In our experience for a batch of 10 cases the HistoGel EtOH protocol takes approximately 50 consecutive minutes whereas the standard HistoGel protocol takes approximately 30 minutes
Add 100 μL of warm Histogel pipet up and down and transfer to the piece of Parafilm leaving it to solidify at 20C25C around 15 min 54 could also be tested such as including DNAse I treatment as previously described by 6 as the general protocol for the expansion of cancer PDOs
1 Prepare HistoGel per manufacturers directions 2 Using a clean pipette add 35 ml of liquid HistoGel Vortex mildly to distribute and place on ice 3 Using a clean wooden applicator stick carefully slide the cell pellet out of tube and into a cassette lined with black biopsy filter paper 4
PDF Thermo Scientific HistoGel Incubator Instructions for Use
1 The recommended longterm storage of HistoGel is refrigerated 28º C This is for overnight storage and storage for when the HistoGel is not in use 2 When using HistoGel during the working day HistoGel may be kept at room temperature 3 When using HistoGel during the working day HistoGel may be kept molten 50 C 5 To Melt
Koch Institute for Integrative Cancer Research Histology Lab 500 Main St 76182 Cambridge Ma 02139 Suggested Method of Applying HistoGel Thermo Scientific to Histology and Cytology Specimens Principle HistoGel is an aqueous gel composition useful in processing very small fixed Histology and Cytology specimens